パルプ中の多糖類に関する研究

第1報　パルプ中の多糖類組成定量のための全加水分解条件の検討

抄録

In order to determine the composition of polysaccharides of pulp, E. Hägglund proposed a procedure of total hydrolysis of pulp, and later various modified procedures of his one were applied by many investigators. In the present paper, occurence and decomposition of monosaccharides during the total hydrolysis have been investigated by means of paper chromatographic technique.
From this investigation, it has been found that a procedure of total hydrolysis is enough to complete the hydrolysis of cellulose, which consists of a primary hydrolysis for 1 hr. at 30°C with 72 % H₂SO₄ and a secondary one for 6 hrs. with boiling diluted (4.0 %) H₂SO₄. But hydrolysis of xylan and mannan had already been completed within 4 hrs. secondary hydrolysis.
When much glucose in hydrolysate interfere the spot of mannose on the paper strip, it is recommended to use glucose oxidase, 10 mg of which can oxidize 10 and 50 mg of glucose within 5 and 20 hrs. respectively (pH 7.0, 37°C).
Water soluble polysaccharides (γ-cellulose) in pulp were completely hydrolyzed by boiling with 4% H₂SO₄ for 8 hrs. In this case hydrolysis of xylan was also easier than that of mannan and glucan.