靱皮繊維の生化学パルプ化機構について
1988 年 42 巻 4 号 p. 330-338
詳細
1988 年 42 巻 4 号 p. 330-338
Mechanistic investigations on biochemical pulping of bast fibers were overviewed from viewpoints of biochemical properties of enzymes causing maceration and solubilized components.
The pulping using pectinolytic enzymes secreted by Erwinia carotovora was found to proceed by maceration as the result of concerted reaction of endo-pectate lyase (endo-PATE) and endo-pectin lyase (endo-PNTE). The endo-PATE was composed of 4 kinds, or sometime 2 kinds, of pI-isozyme, depending on the strains used. The optimal constitution ratio of endo-PATE to endo-PNTE was approximately 30 : 1.
Both in alkaline presoaking and enzymatic pulping, lignin-carbohydrate complexes with molecular weight of 20, 000-25, 000 were found to be eluted.
As to aggregation between pectic substance and LCC, endo-PATE yielded the elution of LCC rich in L-arabinose and D-galactose, while endo-PNTE produced that mainly composed of D-xylose in the pulping of mitsumata (Edgeworthia papyrifera Sieb. et Zucc.). This result was commented in relation with biosynthesis process of hemicellulose.
