Abstract
Colony formation ability of various samples of Eagle’s Minimum Essential Medium (MEM) was examined using two human liver cell lines (OUMS-29 and HLE). OUMS-29 line was derived from human fetal liver cells immortalized with SV40 LT, and HLE line was from human hepatoma Each MEM was supplemented with the same lot of fetal calf serum at a concentration of 10%. MEM produced by N company could hardly sustain colony formation of OUMS-29 cells, while the medium showed good colony formation of HLE cells. On the other hand, MEM produced by M company demonstrated a good efficiency of colony formation for both cell lines. These results indicate that in some cases cell growth may depend on different MEMs produced by various companies, even though every MEM has almost the same ingredients in terms of amino acids, vitamins, and minerals. At present, such a difference in colony formation ability of different MEMs remains to be elucidated.
