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Volume 26 (2007)
- Issue 4 Pages 171-
- Issue 3 Pages 149-
- Issue 2 Pages 115-
- Issue 1 Pages 21-
Volume 26, Issue 2
Displaying 1-4 of 4 articles from this issue
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Jeong Ik Lee, Takashi Kenmochi, Ryohei Nishimura, Hedeaki Sakai, Nobuo ...2007 Volume 26 Issue 2 Pages 115-123
Published: 2007
Released on J-STAGE: July 31, 2007
JOURNAL FREE ACCESSThe chondrocytes of the cartilage in the canine ear pinna were cultured in temperature-responsive culture dishes and a chondrocytic sheet was harvested by lowering the cultural temperature. Two three-layered chondrocytic sheets were used to encapsulate the islets from rats, and a bio-artificial pancreas was made. Histologically the islets were well encapsulated within chondrocytes and was viable based on AZAN staining and immunohistochemistry using anti-insulin antibody. If the chondrocytic sheet may have immunoisolative function, this new type bio-artificial pancreas may be used for the treatment of diabetes with minimum or no immunosuppressive agents.View full abstractDownload PDF (646K) -
Ken SUGO, Tetsuro OGAWA2007 Volume 26 Issue 2 Pages 125-131
Published: 2007
Released on J-STAGE: July 31, 2007
JOURNAL FREE ACCESSThis report describes the primary culture of rat bone marrow cells. The hydroxyapatite (HA) microcarrier was used for the scaffold, which is compared with the HA disk and the T75 flask. Glucose consumption was measured to evaluate cell growth. Much higher glucose consumption was detected on the HA microcarrier group at the early stage of culture than on the T75 flask. This means that the cells on the HA microcarrier proliferated more rapidly. The differentiation potency to the osteoblast was evaluated to determine whether the proliferated cells on the HA microcarrier were still functionally active. The alkaline phosphatase activity and the osteocalcin production were detected, and the cells on the HA microcarrier maintained the differentiation potency to the osteoblasts. The three-dimensional cell culture using HA microcarrier is a useful procedure for clinical research in areas such as on artificial bone.View full abstractDownload PDF (477K) -
Masako MITSUI, Kimiko TAKAHASHI, Katsuyuki KOBAYASHI, Takeshi MATSUOKA2007 Volume 26 Issue 2 Pages 133-142
Published: 2007
Released on J-STAGE: July 31, 2007
JOURNAL FREE ACCESSHFDM-1 is a serum-free culture medium for fibroblasts. The amount of plasminogen activator inhibitor-1 (PAI-1) production from lung fibroblasts is closely related to the severity of the lung diseases. The growth rate of newly isolated human lung fibroblast and the amounts of PAI-1 of the cells were examined. Although the growth of fibroblasts cultured in HFDM-1, with or without 0.5% FCS, was slower than that in M199 containing 10% FCS during the first two or four days, respectively, the cells reached comparable numbers after six days. The expression of PAI-1 mRNA in the fibroblasts cultured with 0.5% FCS HFDM-1 was higher than in the cells cultured with 10% FCS M199. This effect was suppressed by the addition of gefitinib, the EGF signal blocker. There were no significant differences in the cellular PAI-1 expression among all three media. The PAI-1 activity in the culture medium and the fibroblasts’ surface uPA activity were suppressed when the cells were cultured in HFDM-1, with or without 0.5% serum, irrespective of the presence of gefitinib. These results suggested that HFDM-1 reduced the effects of serum on the fibroblasts’ PAI-1 production and activity, and would be a useful tool for the investigation of the lung fibroblasts’ roles in the pulmonary diseases.View full abstractDownload PDF (705K) -
Y. Takemura, K. Yamamoto, N. Asada, M. Namba2007 Volume 26 Issue 2 Pages 143-147
Published: 2007
Released on J-STAGE: July 31, 2007
JOURNAL FREE ACCESSColony formation ability of various samples of Eagle’s Minimum Essential Medium (MEM) was examined using two human liver cell lines (OUMS-29 and HLE). OUMS-29 line was derived from human fetal liver cells immortalized with SV40 LT, and HLE line was from human hepatoma Each MEM was supplemented with the same lot of fetal calf serum at a concentration of 10%. MEM produced by N company could hardly sustain colony formation of OUMS-29 cells, while the medium showed good colony formation of HLE cells. On the other hand, MEM produced by M company demonstrated a good efficiency of colony formation for both cell lines. These results indicate that in some cases cell growth may depend on different MEMs produced by various companies, even though every MEM has almost the same ingredients in terms of amino acids, vitamins, and minerals. At present, such a difference in colony formation ability of different MEMs remains to be elucidated.View full abstractDownload PDF (179K)
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