抄録
We investigated composition and content of glycosphingolipids (GSLs) of rat renal glomerular epithelial cells (SGE1) in vitro, and showed GSLs change during growth and differentiation of the cells. To analyze GSLs, we used a high sensitivity fluorescent labelling method on thin-layer chromatogram. The method could detect GSLs from as little as 107 cells. The GSLs of SGE1 were composed of CMH, CDH, CTH, globoside, paragloboside, Forssman antigen and other minors for neutral GSLs, and GM3, GM2, GM1, GD1a and other minors for ganglioside. We used 2 culture ways; one was monolayer culture and the other was collagen-embedding 3-dimentional (3D) culture that induced cell differentiation. In the case of monolayer culture, GM3 content increased at 6 days after seeding, with increase of γ-GTP activity and with dome formation, the markers for SGE1 cell differentiation. In the case of collagen-embedding 3D culture, content of GD1a of ganglioside, CMH and globoside of neutral GSL as well as GM3 increased, with increase of γ-GTP activity and 3D cyst formation. These results showed that GSLs change of SGE1 cells quantitatively correlated with cell growth and differentiation of renal epithelial cell.
