LOW-FREQUENCY VIBRATORY SOUND INDUCES NEURITE OUTGROWTH IN PC12M3 CELLS IN WHICH NERVE GROWTH FACTOR-INDUCED NEURITE OUTGROWTH IS IMPAIRED

抄録

A drug-hypersensitive PC12 mutant cell line (PC12m3) was obtained during continuous culturing of neural PC12 cells. In this study, PC12m3 cells were exposed to vibratory sound stimuli of frequencies ranging from 10 to 200 Hz for 30 minutes at intensity 5 under the condition of NGF treatment. The results showed that low-frequency vibratory sounds of 10-100 Hz induced enhancement of neurite outgrowth, whereas vibratory sounds 150 Hz and 200 Hz had little effect on neurite outgrowth. The frequency of neurite outgrowth induced by 40-Hz low-frequency vibratory sound stimuli was approximately 3-fold greater than that induced by NGF alone. The activation of p38 MAPK has been shown to play an important role in neuronal differentiation in PC12m3 cells. Therefore, we examined whether the ability of low-frequency vibratory sound stimulus to induce neurite outgrowth of PC12m3 cells is a reflection of its effect on p38 MAPK activity. The results demonstrated that 40-Hz low-frequency vibratory sound stimuli had an enhancing p38 MAPK activity and indicated that vibratory sound induces neurite outgrowth via a p38 MAPK signaling pathway in PC12m3 cells.