Abstract
Eleutheroside E is an active lignan component from Acanthopanax senticosus, which has several biological activities, including anti-fatigue, anti-stress, anti-gastric ulcer, and immunoenhancing effects. However, anti-inflammatory effects have not been examined. In this study, we examined the inhibitory effects of eleutheroside E on the gene expression of inflammatory proteins and transcription factors AP-1 and NF-KB binding activities in IL-1/3 stimulated SW982cells. Eleutheroside E had little cytotoxicity to SW982 cells. Eleutheroside E suppressed the gene expression of IL-6, MMP-1, and COX-2 at lower concentrations than eleutheroside B and isofraxidin. Similar results were obtained on the production of PGE2. In addition, eleutheroside E inhibited MMP-1 promoter activity, transcription factors NF-KB and AP-1 binding activities, while isofraxidin did not inhibit any of the activities. The present results suggest that eleuthroside E may suppress the gene expression of inflammatory proteins through inhibiting NE-KB and AP-1 binding activities.
