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Volume 25 (2006)
- Issue 3-4 Pages 129-
- Issue 2 Pages 105-
- Issue 1 Pages 23-
Volume 25, Issue 3-4
Displaying 1-4 of 4 articles from this issue
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Yoshihisa KOIKE, Soutarou IWAMOTO, Yasuho FUKUMOTO, Fukumi HIRAGAMI, K ...2006 Volume 25 Issue 3-4 Pages 129-136
Published: December 31, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe investigated cellular damage and differentiation of drug- hypersensitive PC12 mutant (PC12m3) cells caused by UVC irradiation. When PC12m3 cells were exposed to UVC irradiation, the frequency of neurite outgrowth rapidly increased in a dose-dependent manner in the presence of nerve growth factor (NGF). The frequency of neurite outgrowth was maximized at 40 J/m2 of UVC irradiation, and this dose of UVC irradiation induced approximately 25-fold greater neurite outgrowth than that induced by NGF alone. However, the cells showed a strong toxicity at this dose of UVC irradiation. We also investigated whether the ability of UVC irradiation stimulus to induce neurite outgrowth of PC12m3 cells is a reflection of its effect on p38 MAPK activity. The results showed that p38 MAPK is strongly activated but that JNK and ERK were not so strongly activated in PC12m3 cells exposed to UVC irradiation of 40 J/m2. Furthermore, UVC irradiation rapidly and strongly activated cyclic-AMP response element (CRE)-binding protein (CREB) in PC12m3 cells. CREB is a transcription factor that is the target of MAPK. These findings suggest that UVC irradiation induced neurite outgrowth through p38 MAPK and CREB pathways in PC12m3 cells.View full abstractDownload PDF (3177K) -
T. Yamazaki, T. Matsumura, T. Tsukiyama, T. Tokiwa2006 Volume 25 Issue 3-4 Pages 137-145
Published: December 31, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSEleutheroside E is an active lignan component from Acanthopanax senticosus, which has several biological activities, including anti-fatigue, anti-stress, anti-gastric ulcer, and immunoenhancing effects. However, anti-inflammatory effects have not been examined. In this study, we examined the inhibitory effects of eleutheroside E on the gene expression of inflammatory proteins and transcription factors AP-1 and NF-KB binding activities in IL-1/3 stimulated SW982cells. Eleutheroside E had little cytotoxicity to SW982 cells. Eleutheroside E suppressed the gene expression of IL-6, MMP-1, and COX-2 at lower concentrations than eleutheroside B and isofraxidin. Similar results were obtained on the production of PGE2. In addition, eleutheroside E inhibited MMP-1 promoter activity, transcription factors NF-KB and AP-1 binding activities, while isofraxidin did not inhibit any of the activities. The present results suggest that eleuthroside E may suppress the gene expression of inflammatory proteins through inhibiting NE-KB and AP-1 binding activities.View full abstractDownload PDF (3144K) -
K. Yamamoto, Y. Takemura, A. Niida, N. Asada, M. Namba2006 Volume 25 Issue 3-4 Pages 147-150
Published: December 31, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe have reported cytotoxic effects of tap water on cultured human liver cells. In the previous paper published in 2005, the culture medium made of tap water and Eagle's Minimum Essential Medium (MEM) was occasionally cytotoxic to human liver cells, indicating that the tap water contained some cytotoxic substances. Our present experiment demonstrated that this cytotoxicity was completely alleviated when Dulbecoo's modified Eagle's medium (DMEM) was used instead of Eagle's MEM. Then we found that a substance in DMEM to prevent the cytotoxicity of tap water was pyruvate.View full abstractDownload PDF (830K) -
Joon-Ho CHO, Takehiro ITOH, Yutaka SENDAI, Hiroyoshi HOSHI2006 Volume 25 Issue 3-4 Pages 151-159
Published: 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSThere are only limited informations available for essential factors which lead to attain complete growth of bovine oocytes derived from early antral follicles. This study was designed to examine factor(s) necessary for achieving full growth of bovine oocytes from early antral follicles. The effect of keratinocyte growth factor (KGF) on the stimulation of the oocyte growth and development was investigated in a culture of cumulus-oocyte complexes with granulosa cells (COCGs) for up to 16 days. A high proportion of COCGs with normal morphology could be maintained even in the control growth medium, and the addition of KGF showed no further increase in the proportion of COCGs with normal morphology. However, the mean oocyte diameter of COCGs was significantly increased in the KGF-containing medium compared to the control after 16 days. After a subsequent in vitro maturation, the in vitro grown oocytes treated by KGF apparently increased the rate of meiotic competence as indicated by an increase in the number of oocytes reaching the M II phase as compared to the control. Furthermore, the in vitro grown oocytes cultured in KGF-containing medium resulted in high rate of developmental competence after in vitro maturation, in vitro fertilization and in vitro embryo culture. These results strongly suggest that KGF may be an important regulator for growing oocytes to reach their full growth potential.View full abstractDownload PDF (2941K)
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