神経栄養因子ガングリオシドと神経伝達アミンとの相互作用

抄録

Ganglioside (GMl) binding octylsepharose (CL-4B) column was prepared for the measurement of an interaction between ganglioside and amines by the method of on affinity chromatography. Every amine had an affinity to this column with an increased el凶 onvolume (PBS-K or PBS-Ca) as compared to the control column (CL-4B only).

The most of these affinities were based on an acid-base interaction because of a linear correlation between the elution volume and the basicity of amine. Serotonin, curare and 3-methoxtyramine had more strong affinities including the specific interactions with glycoconjugates than the acid-base interactions. Acetylcholine and dopamine had the weak affinities resulted from the interfering effects of binding to ganglioside.

This interaction may cause to increase the opportunity of the receptor-binding of neurotrans-amine by the delaying the diffusion of amines from synapse gap.