プロシーディング
In vitroにおけるビオチンのマウス胎児の口蓋突起発育に及ぼす影響
1994 年 11 巻 p. 89-95
詳細
1994 年 11 巻 p. 89-95
The palatal development of mouse embryos on day 12 of gestation was studied by a suspension culture technique of the maxilla. In palatal explants of control embryos after 72 hours in culture, the fusion of palatal processes was significantly increased to about 80% in biotin-supplemented medium (more than 10-8M), as compared with 27 % in the biotin-free medium. Organic acids associated with biotin enzymes such as propionic acid and menthyl crotonic acid, as well as avidin affected the development and fusion of palatal processes. In biotin-deficient palatal explants, the incidence of tha palatal fusion was only 30 % even in biotin-su.pplemented medium (10-6M). The development of biotin-deficient palatal processes was not completely recovered by biotin supplementation and injection. No pathological changes were observed in the palatal processes cultured in biotin-free medium. These findings indicate that biotin plays an important role as a growth factor in the development of palatal processes and/ or that organic acids may interfere with palatal development in biotin-deficient embryos.
