プロシーディング
Participation of Trace-elements in "Function Water" to the restoration of cellular function of Tributyltin chloride intoxicated Euglena gracilis Z
1996 年 13 巻 p. 105-110
詳細
1996 年 13 巻 p. 105-110
In our previous study it has been reported that either Ca or Mg in the "Function Water" prepared by the treatment with high electric field loading in the presence of charcoal or Al-Fe based ceramics should have participated in the restoration of regeneration of flagellum and motility of tributyl tin chloride (TBTCl) -intoxicatedEuglena gracilis Z. In the present study, the effect of "Function Water" prepared by Al-Fe based ceramics material on the ATPase activity of TBTCl-intoxicated E. gracilis Z was examined. The "Function Water" was prepared by immersing one piece of alumina-iron (Al-Fe) based ceramic in the ultra-pure water overnight before examining its effect. TBTCl-intoxication on the Euglena cells was brought about by exposing cells (105 / ml) to 50 μM of TBTCl for 3 minutes. To examine restoration of motility of TBTCl-intoxicated cells by the "Function Water", the cells were washed and subsequently incubated with the "Function Water". Restoration of the cells was evaluated under the microscope equipped with computer aided image analyzer. Effect of "Function Water" on A TPase activity was evaluated by radio-assay using 32P-labelled ATP and E. gracilis Z homogenate prepared from TBTCl-intoxicated Euglena cells or non-intoxicated cells.
Exposure of 50 μM TBTCl to Euglena cells for 3 minutes inhibited ATPase activity by ca. 40%, and incubation of the cells in Al-Fe ceramics treated water definitely recovered ATPase activity to 90% of the control activity in 4 hours. However, the "Function Water" which was treated with either EGT A or Chelex 100 to trap minerals did not show any restoration effect at all, indicating that minerals should have participated in its restoration. Since treatment with Desferal (deferoxamine) specific to iron cherating did not affect the restoration effect so much, the effect of the Al-Fe based ceramics treated water should not be due to iron but either Ca or Mg.
