Effect of oyster extracts on the recovery of cell motility of TBTCl-intoxicated Euglena gracilis Z

抄録

Since oyster is an excellent source of minerals and taurine, it has been regarded as a health food. The authors have studied the biological role of trace minerals on the detoxification of xenobiotics by using tributyltin chloride (TBTCl) -intoxicated Euglena gracilis Z. In the present study, we examined detoxification effects by evaluating the recovery of cell motility for different kinds of oyster extracts; i.e., hot water extract of whole oyster flesh, extract rich in low molecular wt. fraction, extract rich in high molecular wt. fraction, and antioxidant rich fraction. After intoxication with 50 μM TBTCl, E. gracilis Z cells were washed with aqueous solutions containing 4 different oyster extracts separately at the dilution ratio of 100-100, 000-fold, then incubated for 0-180 minutes at 28°C under illumination (2800 lx). Recovery of cell motility was examined under the video microscope. Results showed that hot water extract at 1, 000-fold dilution and the extract rich in the high molecular wt. fraction at 10,000 to 100,000-fold dilution was most effective on the recovery of cell motility. Since the treatment with a chelator, Chelex-100, suppressed the recovery of cell motility by trapping Ca, Mg and Fe in the solution, these minerals seem to have participated in the detoxification of TBTCl and/ or the recovery of cell motility.