グルタチオンと微量金属の結合能 -水銀分析への応用-

抄録

A new method was developed to determine heavy metal ions taking advantage of the selective ion fixations. The principle is to measure the decrease of GSH by the fixation. GSH were measured by NAM fluorometry in the concentration range between 10^-9~10^-12 mol/ml. The decrease of the fluorescence was proportional to the metal ion concentrations. The selectivities of the ions were controlled by EDTA, Cl^-, pH and ion strength. Hg⁺, Hg²⁺ and CH₃Hg were selectivelly determined. The other ions were completely masked by the EDTA solution.