The effects of Crassostrea gigas extract (JCOE) on endoethial injury were investigated using a cell line HAEC, human aortic human aortic endothelial cell. Cells were subjected to hypoxia in a chamber with 95% nitrogen and 5% carbon dioxide for 4 hours. Reoxygenation was initiated by replacing the media and putting the cells in an environment of room air plus 5% carbon dioxide. Anoxia-reoxygenation induced cellular oxidative stress, which was determined by the levels of reduced glutathione as well as the levels of intracellular reactive oxygen species. Cell viability of HAEC cells subjected to anoxia-reoxygenation significantly decreased. Preteatment of JCOE for 24 hours at a dose of 1000 μg/ml siginificantly reversed the decrease of cell viability induced by anoxia-reoxygenation,and also reversed the decrease in cellular glutathione levels. These results show that JCOE contains one of effecting functional food factors for reducing the oxidative stress in endothelial cells.
