There are only a few data which have been reported concerning the dietary intake of biotin in the Japanese population. The biotin intake in the adults was 29.833.3 g/day in a geometric mean by Duplicate Meals and 54.3 μg/day in an arithmetical mean after re-analysis. We also developed the new dietary survey Food Groups Calculation Study. Biotin intakes estimated by Food Groups Calculation Study (18 food groups) was 109.8 μg/day for men and 92.3 μg/day for women. However, the biotin intakes using Food Groups Calculation Study (98 food group) was 54.5 μg/day for adults. Total Diet Study gives the result of 50.7 μg/day. From these findings, it is suggested that the estimated daily intake of biotin for adults is 50 μg/day by these dietary surveys in Japan.