Toenail samples were collected from 62 healthy subjects (36 males and 26 females, 25.2 ± 9.6 yr) in Osaka Prefecture of Japan and their selenium contents were determined by inductively coupled plasma-mass spectrometry and compared to those described in several previous reports. Maximum, minimum, mean, standard deviation and median values of the selenium in 62 toenail samples were 2.13, 0.18, 0.77, 0.32 and 0.77 μg/g, respectively. The mean value obtained was similar to values in the eastern and western USA area and lower than values in a seleniferous area of the USA and higher than values in New Zealand, Finland and most European countries where the selenium intake were about 50 μg/d or less. These results indicate that toenail selenium is a good indicator for averaged selenium intake.