亜セレン酸に曝露されたインゲン、リョクトウ、およびアズキのスプラウトにおけるセレノホモランチオニンの蓄積

抄録

Sprouts of common beans, mung beans, and azuki beans were cultivated in an environment exposed to selenite at a level of 1, 5, 10 or 20 µg Se/mL, and the molecular species of selenium produced were analyzed by high performance liquid chromatography with inductively coupled plasma mass spectrometry. Selenohomolanthionine (SeHL), Se-methylselenocysteine (MSeC), and selenomethionine (SeM) were identified in the sprout of all beans at 20 µg Se/mL exposure. The amount ratio of the three selenoamino acids was about 1/1/0.5 for SeHL/MSeC/SeM in common beans, but SeHL accounted for most of the selenium molecular species in mung beans and azuki beans. When the exposure concentration was 10 µg Se/mL or less, the amount ratio was the same as in the case of 20 µg Se/mL in common beans and azuki beans, but in mung bean, an amount of MSeC was close to that of SeHL. These results indicate that SeHL is the major selenium metabolite in sprouts of common beans, mung beans, and adzuki beans exposed to selenite, and that these bean sprouts have a low ability to methylate selenol.