Determination of Tin in Biological Samples by Gaseous Hydride Generation-Atomic Absorption Spectrometry

抄録

A highly sensitive tin determination method for biological samples is presented. Tin reduced to gaseous hydride by sodium tetrahydroborate solution and trichloroacetic acid solution was atomized through an electrically heated silica tube and measured by atomic absorption spectrometry at a wavelength of 286.3 nm. Sample decomposition procedure was avoided in determining tin in urine in order to avoid contamination with tin tin acids used for sample digestion. Recovery of tin was 80% at 1:5 dilution of human urine and was 102% at 1:10 dilution. The relative detection limit of urine tin was 2.0 ng/ml, that of standard tin solution was 0.3 ng/ml and urine was diluted 5-fold to determine tin concentration. A 10ml sample solution is necessary for determining of tin. The coefficient of variation of human urine tin determination was 1.6% at 10 ng/ml. The method can be applied to determination of tin concentration in biological samples.