p- ヒドロキシアセトフェノンの酵母増殖阻害回復機構の解明

抄録

Plants are an important source of anti-inflammatory and antioxidant compounds. The antifungal action of p-hydroxyacetophenone (p-HAP) was reported in the manuscript investigating the antifungal activity of 2’,6’-dihydroxy-4’-methoxyacetophenone isolated from Ezomurasaki azalea leaves and taken up as a related compound. Further, when producing bioethanol using yeast, p-HAP exhibits growth inhibitory effects due to the p-HAP contained in raw materials such as wheat, which reduces the amount of ethanol produced. However, the pharmacological action of p-HAP on yeast has not been clarified in detail, so we attempted to examine it in this study. We extracted and identified three proteins using p-HAP-sepharose affinity chromatography. Among these three, we focused on NADPH dehydrogenase in particular and examined the effect of supplementation of NADP⁺. As a result of this study, we found that NADP ⁺ was effective in restoring the growth of the yeast. Assuming that the 15mM p-HAP as the NADP⁺ non-addition group was 100%, the effects were 136% ( 2.5mM NADP⁺, 15mM p-HAP addition group), 107% ( 1.25mM NADP⁺, 15mM p -HAP addition group ) and 102% ( 0.63 mM NADP⁺, 15 mM p-HAP added group) at 12 hours. At 25.5 hours, it was also elevated to 143% ( 2.5 mM NADP⁺, 15 mM p-HAP added group ), 113% ( 1.25 mM NADP⁺, 15 mM p-HAP added group) , and 104% ( 0.63 mM NADP⁺, 15 mM p-HAP added group) in a concentration dependent manner. Moreover, significant differences ( p < 0.01 or p < 0.05 ) were confirmed in the 2.5 mM NADP⁺, 15 mM p-HAP addition group from the 15 mM p-HAP addition group at all measurement times. Therefore, it is expected that the supplementary addition of NADP⁺ would partially recover the growth of the p-HAP-added group in the yeast.