電子スピン共鳴法による血中バナジウムのモニタリング

抄録

Physiological roles of vanadium have extensively been studied, and various functions of this metal ions are proposed. Vanadium ion is usually present as the oxidation states of +3, +4or +5, thus such oxidation states are expected to relate their biological functions. We investigated whether the blood vanadium levels can be monitored when vanadium compounds with various oxidation states are given to animals. Vanadium compounds were injected to rats intrapenitorially at the dose of 5 mg/kg body weight as VCl₃ VOSO₄ or NaVO₃. Total vanadium and vanadyl (+4 oxidation state) levels in blood and serum were determined by neutron activation analysis (NAA) and electron spin resonance (ESR) method, respectively. The vanadium concentrations determined by NAA and ESR were in good agreement. Blood vanadium levels after 1 hr of administration of the compounds were found to be the highest in rats treated with V(+5)compound among three compounds and followed by V(+4) and V(+3) compounds. Blood vanadiums in rats treated with V(+5)and V(+4)compounds were present exclusively as +4 oxidation state (VO²⁺). Thus the blood vanadium levels were monitored by ESR on rats treated with vanadium compounds and the data were analyzed pharmacodynamically.