The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
Original Article
CONTRIBUTION OF INTESTINAL CALCIUM ABSORPTION TO 1,25-DIHYDROXYVITAMIN D3-INDUCED CALCIUM ACTION IN THE TOTAL PARENTERAL NUTRITION RAT
Kentaro ASANUMAShun-ichiro KOMATSUTakayuki SAKURAIRyo TAKAIShuichi CHIBA
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2006 Volume 31 Issue 4 Pages 325-344

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Abstract

To investigate the contribution of intestinal calcium (Ca) absorption to 1,25-dihydroxyvitamin D3 (1,25(OH) 2D3)-induced Ca action, we assessed parameters related to Ca metabolism after a single dosing of 1,25(OH)2D 3 in the total parenteral nutrition (TPN) solution or 5% D-mannitol (MAN) solution treatment with rats. Animals were divided into 6 groups (vehicle, 100 μg/kg p.o. and 25 μg/kg i.v.; n=8) in Experiment 1 and 8 groups (vehicle, 1, 10 and 100 μg/kg p.o.; n=6) in Experiment 2 at TPN or MAN solution treatment. In both experiments, the parameters related to Ca metabolisms, urinary Ca and urinary deoxypyridinoline on 0-24 hr or serum Ca, osteocalcin and parathyroid hormone at 24 hr were measured after 1,25(OH)2D 3 dosing. 1,25(OH)2D3-related increased urinary Ca or serum Ca were observed in both experiments. Decrease rates in change of urinary Ca in TPN solution treatment rats were 36.3% (100 μg/kg p.o.) or 47.1% (25 μg/kg i.v.) of MAN solution treatment rats in Experiment 1, and 29.0% (1 μg/kg), 56.2% (10 μg/kg) or 35.3% (100 μg/kg) of MAN solution treatment rats in Experiment 2. Decrease rates in change of serum Ca at 72 hr in TPN solution treatment rats were 57.3% (100 μg/kg p.o.) or 44.5% (25 μg/kg i.v.) of MAN solution treatment rats in Experiment 1, and were 57.0% (100 μg/kg) of MAN solution treatment rats in Experiment 2. There were no differences in the change of serum Ca in the 1,25(OH)2D3 1 or 10-μg/kg group in Experiment 2. Our results suggest that differences in urinary Ca or serum Ca between MAN solution treatment rats and TPN solution treatment rats express the contribution of intestinal Ca absorption to 1,25(OH)2D3-induced Ca action in the conditions of the study.

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© 2006 The Japanese Society of Toxicology
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