The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
Original Article
CHARACTERIZATION OF AN IMMORTALIZED HEPATIC STELLATE CELL LINE ESTABLISHED FROM METALLOTHIONEIN-NULL MICE
Nobuhiko MIURAYoshitaka KANAYAMAWakako NAGAITatsuya HASEGAWAYoshiyuki SEKOToshiyuki KAJIAkira NAGANUMA
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2006 Volume 31 Issue 4 Pages 391-398

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Abstract

Hepatic stellate (HS) cells were isolated from the livers of metallothionein (MT)-null and control mice and used to establish IMS/MT(-) and IMS/N cell lines, respectively, using SV40 virus transformation. Cellular morphology, incorporation of vitamin A and expression of α-SMA, desmin and SV40 T-antigen were used to confirm that both cell lines were immortal HS cells. The growth rates of both cell lines were similar and there was little difference between cell line sensitivity to zinc. MT-null IMS/MT(-) cells were more sensitive to cadmium and mercury, although both cell lines accumulated almost equal amounts of cadmium during a 24-hr culture period. As HS cells play an important role in hepatic fibrosis and are activated by heavy metals such as cadmium or reactive oxygen, the MT-null HS cell line derived in this study should be a useful experimental model for examination of the role of MT in HS cell activation.

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© 2006 The Japanese Society of Toxicology
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