The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
Original Article
OPTIMIZATION OF AN ANIMAL TEST PROTOCOL FOR TOXICOGENOMICS STUDIES (I); REQUIREMENT STUDY OF A PROTOCOL
Kayo SUMIDAKoichi SAITOKenji OEDAMasanori OTSUKAKazunari TSUJIMURAHideki MIYAURAMasaru SEKIJIMAKoji NAKAYAMAYukiko KAWANOYuki KAWAKAMIMakoto ASAMOTOTomoyuki SHIRAI
Author information
JOURNALS FREE ACCESS

2007 Volume 32 Issue 1 Pages 19-32

Details
Abstract

Toxicogenomics is a promising new tool for prediction of chemical toxicities including carcinogenicity in a relatively short period. However, it is important to develop a reliable animal test protocol for toxicogenomics studies. The preparation of RNA and tissues is also crucial, since it greatly influences outcomes of gene expression analysis.
In the present study, we examined an animal test protocol by comparing gene expression data from different conditions and proposed a reliable animal test protocol for toxicogenomic studies.
With regard to the preparation of tissues and RNA, here we present evidence that quality of RNA and tissues is well-preserved even after freezer storage for up to 2.5 years. Gene expression levels were compared using a GeneChip System (RGU34A, Affymetrix, Santa Clara, CA, USA) between RNA samples that were freshly prepared, stored at −80°C or re-prepared from tissue kept at −20°C. None showed degradation and no significant differences in expression were evident among the three sets of samples. The data demonstrate that gene expression analysis by DNA microarray is suitable for RNA or tissues that have been stored at an appropriate temperature.

Information related to the author
© 2007 The Japanese Society of Toxicology
Previous article Next article
feedback
Top