The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
Letter
Tributyltin induces cell cycle arrest at G1 phase in the yeast Saccharomyces cerevisiae
Takayuki SekitoNaoko SugimotoMasaya IshimotoMiyuki Kawano-KawadaKoichi AkiyamaSogo NishimotoTakuya SugaharaYoshimi Kakinuma
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2014 Volume 39 Issue 2 Pages 311-317

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Abstract

Tributyltin (TBT) has long been recognized as a major environmental pollutant that can cause significant damage to the cellular functions as well as disruption of endocrine homeostasis. TBT induces apoptosis accompanied by production of reactive oxygen species (ROS) in mammalian and yeast cells. We observed that the budding yeast cells exposed to this compound at low concentrations exhibited cell growth arrest, but not cell death. Flow cytometric analysis of yeast cells without synchronization and morphological assessment of cells synchronized at M phase by nocodazole treatment indicated that TBT-exposed Saccharomyces cerevisiae cells were arrested at G1 phase of the cell cycle. This arrest was recovered by the addition of N-acetylcysteine, suggesting the involvement of ROS production by TBT. This is the first study to evaluate the action of TBT on cell cycle events.

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© 2014 The Japanese Society of Toxicology
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