The Journal of Toxicological Sciences
Online ISSN : 1880-3989
Print ISSN : 0388-1350
ISSN-L : 0388-1350
HIGH SUSCEPTIBILITY TO AFLATOXIN B1 AND BENZO[A]PYRENE OF BALB3T3 A31-1-1 CELLS EXPRESSING MONKEY CYP1A1
Susumu ITOHSanae TAGAWAMinoru SAWADATetsuya KAMATAKI
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Keywords: Cytotoxicity
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1993 Volume 18 Issue 3 Pages 207-210

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Abstract

The monkey CYP1A1 has been expressed in BALB 3T3 A31-1-1 cells and the expressed proteins were assayed for their capacity to activate aflatoxin B1(AFB1) and benzo[a]pyrene (B[a]P). The transformed cells were approximately 5.4-and 4/7-fold more sensitive to AFB1 and B[a]P than the parental cells, respectively. These results indicate that monkey CYPlA1 cDNA encoded a functional protein and that the expressed CYPlAl enzyme is active for the activation of B[a]P as well as AFB1 to produce toxic metabolites.

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