CHARACTERISTICS OF PERIPHERAL BLOOD MONOCYTES AND BONE MARROW MACROPHAGES FROM RATS TREATED WITH MITOMYCIN C, 5-FLUOROURACIL OR PHENYLHYDRAZINE

Abstract

We examined the cellular functional changes in bone marrow macrophages dbtained from rats treated intraperitoneally with mitomycin C (MMC), subcutandously with 5-fluorouracil (5FU) or intraperitoneally with phenylhydrazine (PHZ) for 7 days to define the mechanisms of toxicity in the bone marrow. The functions of peripheral blood monocytes and bone marrow macrophages were also compared. MMC and PHZ caused anemial and MMC and 5FU leukocytopenia. Changes in nucleated cell counts were different among the three drug-treatment groups, and MMC- and PHZ-treated bone marrow macrophages were activated for responsiveness to interleukin-1 (IL-1), and their migration activity, intracellular components and enzyme activities were increased. On the other hand, cellular functions of bone marrow macrohhages of rats treated with 5FU were suppressed.