Production of Hybridoma Secreting Specific Antibody against Horseradish Peroxidase Isozyme

Abstract

The specific antibody against horseradish peroxidase isozyme was prepared by a cell fussion technique between peroxidase-immunized mouse splenocyte and myeloma, P3-X63-U1 AZG^R. The prepared antibody could specifically bind to Type VIII isozyme and repress its activity. In contrast, the conventional antiserum could not differentiate the specific antigen sites of isozymes.