2011 Volume 64 Issue 6 Pages 453-456
We investigated the effects of immunization against bovine viral diarrhea virus (BVDV)-2 with a two-step vaccination program using attenuated live BVDV1 after immunizing by inactivated BVDV1 and BVDV2 vaccine. Fifteen calves that had taken adequate colostrum from a dairy farm in the Nemuro District of Hokkaido, Japan,were used. The calves were divided into three groups: Group A was inoculated with one dose of inactivated BVDV1 and BVDV2 vaccine. Group B was inoculated with inactivated BVDV1 and BVDV2 vaccine at first and then inoculated with a live vaccine containing BVDV1 (a two-step vaccine program). Group C was inoculated with only one dose of live BVDV1 vaccine. The antibody titer against BVDV2 in group B was significantly higher than that in group A and C after inoculation with the live BVDV1 vaccine. This result suggested that the two-step vaccine program using in this study is useful for enhancing antibody titers against BVDV2.
