Abstract
An experimental inoculation of Psittacine beak and feather disease (PBFD) virus (PBFDV) was implemented to reproduce the peracute form of PBFD. Five newly hatched cockatiels free of PBFDV antibodies were inoculated intramuscularly with the viral emulsion, and investigated histopathologically at 1, 3, 4, 6, and 10 weeks after inoculation. Also, etiological examination with PCR analysis of tissues from various organs was performed. Although the results of PCR showed that infection with PBFDV was established after three weeks, the clinical signs and histopathology were consistent with those of acute PBFD rather than those of peracute PBFD. These results suggest that newly hatched cockatiels infected with PBFDV showed the acute form of PBFD, and that experimental reproduction of the peracute form may be difficult.
