Abstract
Eight healthy Holstein dairy cows were inoculated with an inactivated bovine salmonellosis vaccine that was composed primarily of inactivated somatic antigens of Salmonella typhimurium and Salmonella enterica serovar Dublin and contained polymixin B sulfate, an endotoxin neutralizer. Clinicopathologic responses were observed. After vaccination, neither a decline in appetite nor deterioration in their general condition was noted. Swelling and induration were recognized at the inoculation site. A hematological examination revealed significant post-vaccination changes in the hematocrit, albumin-to-globulin ratio, total protein concentration, aspartate aminotransferase activity, creatine kinase activity and sialic acid concentration in the serum, all of which are markers of acute inflammation. However, no clinical manifestations suggestive of endotoxic shock or anaphylactoid reaction were noted after the vaccination. Although this vaccine causes local acute inflammation at the inoculation site, the likelihood of severe side-effects is low. Therefore, the vaccine can be used effectively in clinical settings.
