2022 Volume 75 Issue 7 Pages e150-e156
In Japan, the tube agglutination test and the complement fixation test have been adopted as serological diagnosis for swine brucellosis since April 2022. However, there is little information on nonspecific reactions and anti-complement activity, which may hinder judgment in both the tests. In this study, therefore, both tests were conducted using serum collected for the inspection of breeding male stock based on the Act on Improvement and Increased Production of Livestock. In the tube agglutination test, nonspecific agglutinations in 32 out of 109 sera (29.4%) were observed, and two serum samples were determined to be suspicious (specificity: 98.2%). In the complement fixation test, eight out of 92 sera (8.7%) showed inhibition of hemolysis that were considered to be the anti-complement activity, of which five sera were determined as not-negative (specificity: 94.6%). These results would be helpful in conducting both the tests in diagnosis of swine brucellosis.
