1968 Volume 21 Issue 2 Pages 73-76
Mucous membranes were collected from the small intestines of pigs infected with the Ukiha and Aso strains of transmissible gastroenteritis virus and made to emulsions, which were inoculated into swine kidney cell culture for serial passage. As a result, both strains began to show cytopathogenic effect (CPE) at the seventh passage. The growth curve of this virus in swine kidney cell culture revealed that viral growth began already at 24 hours and reached a maximum (106.33TCID50/0.1 ml) at 60 hours.
The virus was inactivated with ether, chloroform, and sodium desoxycholate. It decreased a little in infective titer when treated with trypsin. It was unstable to heating, although it was stable at low temperature. It passed through a millipore filter at more than 100 mμ, c, but not one at 10 to 50 mp.It gave rise to CPE in swine kidney and testis cell cultures, but did not in culture of any other cells of animal origin.
Clinical infection with an increase in neutralizing antibody was observed in pigs inoculated with viral materials from cultures at the 17th and 45th passage of virus. No clinical infection was seen in pigs inoculated with viral material at the 70th passage, in which the production of neutralizing antibody was low.
