1983 Volume 36 Issue 6 Pages 325-329
A survey was conducted on the pollution of water with pyrogens by the routine pyrogen test (PT), Limulus test (LT), and general bacterial count (BC).
In PT, 10 ml/kg of undiluted tap or well water was pyrogenic when injected, into rabbits. It was also positive for LT. BC was 0-103/ml for tap and well water.
In PT, 1 and 10 ml/kg of undiluted river and sea water were pyrogenic when injected into rabbits. LT was positive when applied to undiluted river and sea water and dilutions up to ten times. BC was 103-106/ml for river water and 10-103/ml for sea water.
Rain and snow water were negative for PT and LT even when undiluted. BC was less than 102/ml for rain water and less than 10/ml for snow water.
When tap water was heated at 100°C for more than 10 minutes or at 121°C for more than 5 minutes, it became negative for PT. Tap water became negative for PT when filtered through a membrane filter 0.45μm in pore diameter or an asbestos filter plate 0.08μm in pore diameter.