Abstract
The reversed passive hemagglutination (RPHA) test was carried out for detection of canine parvovirus (CPV) in fecal specimens from dogs experimentally and naturally infected with CPV. Glutaraldehyde- and tannic acid-treated sheep red blood cells were sensitized with anti-CPV rabbit serum purified by affinity chromatography. The following results were obtained.
1) In experimentally infected animals, the RPHA positive reaction was observed in the feces, and the titer increased with developing clinical signs of the disease. CPV was isolated from these fecal specimens by inoculation of materials to culture cells.
2) In naturally infected animals, all the fecal specimens with a hemagglutination (HA) titer over 1: 32 were positive for the RPHA test.
3) The specificity of the RPHA test was confirmed by using sheep red blood cells sensitized with CPV-negative serum, instead of positive serum.
From these results, the RPHA test was considered to be a useful diagnostic method for CPV infection because of its specificity, sensitivity and rapidity.
