Eleven Thoroughbred colts were fed tylosin (0.88g per head) mixed with feed once (for 6 colts) or twice (for 5 colts). They began to show apathy and anorexia 1-2 days after the first administration, and diarrhea one day later. Then 3 adulthorses were fed the residual medicated feed. They exhibited the same signs. Four colts died 2-3 days after the first administration. Autopsy revealed mild lesions in the gastrointestinal tract. Microscopically, there were no changes of infection. No pathogenic bacteria were isolated from any organ or the intestinal contents.
A method was devised to treat pulmonary edema secondary to the venae cavae syndrome. In it, a percutaneous biliary drainage tube was inserted from the level of ringlike tracheal cartilage, Then oxygen was administered through a percutaneous biliary drainage tube. This method was effective and safe.
A total of 11, 082 cows with vagus indigestion were surveyed. Bradycardia was not a characteristic finding of any special disease. It was exhibited particular in cows with atony of the forestomach and abomasal impaction. Abomasal impaction, calf intestinal catarrh and cecal dilatation were directly connected with death. The cause of bradycardia seemed to be related not only with irreversible impairment of the vagus nerve, but also with some complicated factors.
Haemophilus somnus infection occurred in a 6-year-old Holstein dairy cow in Gifu Prefecture in June, 1984, a month after delivery. The cow was condemned within a day. Opisthotonus and clouding of consciousness were noticed. When the cerebrospinal fluid was examined, its pressure was high and its color yellowish red. The count of floating cells was 198/mm3. Pandy's reaction was four plus. The concentration of total protein was 100mg/dl, glucose 80mg/dl, inorganic phosphorus 2.7mg/dl, and calcium 7.0mg/dl. An increase in staff neutrophils was seen. Microscopy revealed hemorrhagic meningoencephalitis, thrombosis, vascular degeneration, and perivascular necrosis of the brain and subdural and subarachnoideal hemorrhage of the spinal cord. H. somnus was isolated from the brain and the spinal cord. It was very sensitive in vitro to aminobenzyl-penicillin and oxytetracycline. The titer of the micro-plate agglutination test against H. somnus was 1: 1, 280.
An outbreak of “hyena disease” in cattle was recognized on a farm that had bredabout one thousand Holstein cattle, in Iwate Prefecture over a period from June through August, 1984. Of 82 calves of almost the same age, 40 were affected. They exhibited a low growth rate marked in the hindquarters and defective locomotion which became apparent at about six months of age. Pathological and serological examination were carried out in fourteen cases. Autopsy revealed shortening of the femur and tibia. The shortened femur was mostly accompanied with flattening of the head, as well as twisting of the head and neck. Shortening of the humerus and flattening of its head were also recognized in all the four cases examined. The epiphyseal cartilaginous plates of the femur, tibia and humerus partially disappeared or deformed. Similar lesions were seen also in the vertebra, although only two cases were examined. Low growth of skeletal muscle was noted in the hind legs. Histologically, the resting zone of cartilage of the epiphyseal cartilaginous plate was thickened. Cartilaginous cells decreased in number and disappeared in the proliferative zone. Excessive infiltration of osteoblasts and osteoclasts was seen. Perivascular cuffing of small round cells in the central nervous system was found in 7 cases. Infiltration of small round cells was noticed in the neurohypophysis of 2 cases and in the heart of 6 cases. Neutralizing antibodies against bovine viral diarrheamucosal disease virus were demonstrated in the serum of 5 cases.
The reversed passive hemagglutination (RPHA) test was carried out for detection of canine parvovirus (CPV) in fecal specimens from dogs experimentally and naturally infected with CPV. Glutaraldehyde- and tannic acid-treated sheep red blood cells were sensitized with anti-CPV rabbit serum purified by affinity chromatography. The following results were obtained. 1) In experimentally infected animals, the RPHA positive reaction was observed in the feces, and the titer increased with developing clinical signs of the disease. CPV was isolated from these fecal specimens by inoculation of materials to culture cells. 2) In naturally infected animals, all the fecal specimens with a hemagglutination (HA) titer over 1: 32 were positive for the RPHA test. 3) The specificity of the RPHA test was confirmed by using sheep red blood cells sensitized with CPV-negative serum, instead of positive serum. From these results, the RPHA test was considered to be a useful diagnostic method for CPV infection because of its specificity, sensitivity and rapidity.