Creatine Kinase and Its Isoenzymes in Dogs

Abstract

The mean value and normal range of serum creatine kinase (S-CK) activity were 27.3 IU/L and 8-72 IU/L, respectively, in 81 house dogs of various breeds, 26.1 IU/L and 13.0-41.0 IU/L in 62 beagles. The S-CK activity of the puppy showed a tendency to be higher than that of the adult. Three isoenzymes (CK-MM, CK-MB, and CK-BB) were recognized in serum. Their mean percentages and normal ranges were as follows: 46.3% and 9.6-83.0% for CK-MM, 8.2% and 3.5-16.7% for CK-MB, and 38.5% and 3.8-73.3% for CK-BB in the house dogs and 45.3% and 20.0-70.3% for CK-MM, 10.1% and 4.6-14.7% for CK-MB and 30.5% and 14.7-46.4% for CK-BB in the beagles. One or two sub-bands that differed from the three isoenzymes in electrophoretic mobility were found near CK-MM in 61 of 143 dogs.
All the tissues had CK activity. Skeletal muscle abounded with CK-MM. Cardiac muscle had CK-MM and always had few CK-MB. Smooth muscle had all of the three isoenzymes. Brain abounded with CK-BB. Other tissues had low activity, and specific isoenzyme pattern.
The half life period was 122.0 minutes (SD=23.9) for CK-MM, 125.7 minutes (SD=22.7) for CK-MB, and 49.7 minutes (SD=11.0) for CK-BB in circulating blood.