1987 Volume 40 Issue 8 Pages 557-561
Insulin in bovine blood was determined by two enzyme immunoassay kit (EIA) methods, which were EIA-1 as non-competitive and EIA-2 as competitive method. There were highly significant statistical correlations among the values obtained by these methods and the radio immunoassay (RIA) method. The correlation coefficiant and regression equation were r =0.962 and y=6.5+0.7786 x between EIA-1 and RIA methods, and r =0.908 and y=21.34 + 1.1219 x between EIA-2 and RIA methods. The EIA method seemed to be useful for bovine blood insulin assay in the field. The plasma concentration of insulin was 20.9± 18.66 μU/mi in 125 healthy adult cattle when determined by the EIA-1 method. It was 42.04±19.55 μU/ml in fattening cattle, 10.10±3.24 μU/ml in grazing cattle and 10.88±5.04 μU/ml in dairy cattle.
The level of insulin was apparently higher during and immediately after meal than before meal.
