Insulin in bovine blood was determined by two enzyme immunoassay kit (EIA) methods, which were EIA-1 as non-competitive and EIA-2 as competitive method. There were highly significant statistical correlations among the values obtained by these methods and the radio immunoassay (RIA) method. The correlation coefficiant and regression equation were r =0.962 and y=6.5+0.7786 x between EIA-1 and RIA methods, and r =0.908 and y=21.34 + 1.1219 x between EIA-2 and RIA methods. The EIA method seemed to be useful for bovine blood insulin assay in the field. The plasma concentration of insulin was 20.9± 18.66 μU/mi in 125 healthy adult cattle when determined by the EIA-1 method. It was 42.04±19.55 μU/ml in fattening cattle, 10.10±3.24 μU/ml in grazing cattle and 10.88±5.04 μU/ml in dairy cattle. The level of insulin was apparently higher during and immediately after meal than before meal.
Seven nursing calves of the Japanese Black breed 20 to 87 days of age and one Holstein heifer calf 311 days of age were affected suddenly with myopathy accompanied with stiff gait or recumbency and myoglobinuria 2 to 14 days after turned out to pasture. Clinically, white nuscle disease was diagnosed in the calves, and paralytic myoglobinuria in the heifer calf on the basis of age difference. All the animals showed a marked increase in serum enzyme activities (GOT, GPT, LDH and CPK) and a marked decrease in serum selenium (18.3 ± 10.8 ppb in average) and tocopherol (46.3±29.6μg/dl on the average). In the contents of feedstuffs supplied selenium showed a markedly low level less than 0.1 ppm in the stage of housing and grazing, and dl-α-tocopherol a low level only in the stage of housing. Sodium selenite and dl-α-tocopherol acetate were administered intramuscularly to all the animals for treatment, and seven of the eight calves recovered. It was considered that both white muscle disease and paralytic myoglobinuria in calves might be included in myopathy of the same category caused mainly by deficiencies of selenium and tocopherol.
By the way of infusion with contrast medium (Endografin (R)) through the cervix into the uterus with a balloon or metal catheter, a contrast radiograph could be obtained easily frombitches in the proestrous-estrous or postpartum stage, because their cervix were opened. In bitches in the anestrous or diestrous stage the infusion was achieved by injection with the contrast medium into the uterus directly by laparotomy, because their cervix were closed tightly. In proestrus-estrus, the uterus was the longest of all the stages and widerthan in anestrus. Moreover, it showed many large curves. In diestrus, the shape of the uterus was like spiral spring, cork screw or drill. The contrast radiograph in anestrus showed the uterus narrow in width and straight slender in shape. The movement of the uteruswas not remarkable. In postpartum, the uterus showed various shapes according to the repairing process after delivery. The endometrial outline presented numerous laminated folds inthe early stage, many indentations in the middle stage, gradual diminution in the entire volume of the uterus and a relatively smooth surface in the last stage of the repairing process.
Toxascaris leonina was detected from 8 Japanese-breed domestic cats in Kanagawa Prefecturefrom October, 1983 to March, 1985. Feline infections had hardly been reported in Japan. Cat 1 had been kept indoor out of contact with imported or purebred cats. The ascarids of the cat seemed to be of the “dog strain”, because of their high and low infectivity to dogs and cats, respectively. Cats 2-7 were kept together on the same farm and got incontact with imported cats. With ascarid eggs from one of those cats, dogs and cats wereeasily infected, so that the ascarids seemed to be of the “cat strain” which wouldhave been introduced by imported cats and maintained on the farm. The history of Cat 8 wasnot determined, but its ascarids might be of the “cat strain” because oftheir high infectivity to both dogs and cats. Milbemycin D was administered orally to 3and2 naturally infected cats in doses of 0.05 and 0.1 mg/kg, respectively to deworm all the animals completely, except one administered with 0.05 mg/kg from which young adult worms were recovered at autopsy.
A Japanese Black Ox showed pyrexia, anorexia and depression on a farm in Gunma Prefecture in December, 1985. Autopsy revealed hepatic lesions consisting of numerous approximately spherical pale lesions scattered on the surface. Inflammatory lesions were seen in the right lobe of the lung. A part of the trachea was whitish and thickened. Histologically, necrotic foci were observed in most areas of the liver with proliferation of fungi around blood vessels. The pulmonary lesions were seriously exudative. Fungi were also observed in a part of the bronchiole and vascular wall. Mortierella wolfii was isolated from the liver. The appearance of mycelia in the liver was identical.
On a hog farm of breeding and raising in the Iburi district of Hokkaido, Bordetella bronchiseptica (BP) was isolated from the nasal cavity of breeding sows on the day of parturition and for the subsequent 15 days. Then intranasal spraying with kanamycin sulfate (960 mg titer per capita) was performed in 6 breeding sows 3 days before the expected day of parturition, on the actual day of parturition, and 1, 2, and 3 weeks after parturition. In addition, animal barns were disinfected with chloride of potassium isocyanurate twice a week. As a result, no BP was detected from the nasal cavity of 30 young born from these sows at the time of weaning. Turbinal atrophy was reduced to a larger extent in the treated group than in the untreated control one.
Three piglets 36 days old of the same litter were administered experimentally with cyclophosphamide. One of them died suddenly ofActinobacillus suisinfection. It was made clear that cyclophosphamide impaired the defense mechanism of the animal by suppressingthe immune function. Such immunosuppressive condition was considered to be closely relatedwith opportunistic in fection.
The densities of the separating fluid (Ficoll-Conray) of 1.073-1.077 g/ml were suitable for isolation of lymphocytes from the blood of neonatal calves. The mitogen-induced blastogenic response of lymphocytes isolated from 7 newborn calves was satisfactory when mea suredby DNA fluorometric assay.
Spontaneous systemic lymphosarcoma was found in a 5-year-old Corriedale sheep, showing a marked weight loss. Necropsy revealed a huge tumor located in the anterior mediastinum associated with enlargements of the cervical lymphonode. There were milky white masses in the pars costalis of the diaphragm, jejunum, lymphonodi jejunales, and liver. Histologically, large round tumor cells exhibited a diffuse proliferation, containing a round to irregular-shaped nucleus with indentation or distortion. This case was diagnosed as thymic, diffuselymphosarcoma of large cell type.