New Methods for Isolation of Pathogenic Bacteria from Milk

Abstract

Two methods for isolation of pathogenic bacteria from milk were developed. Both were able to make a small culture volume of a large quantity of milk, and recover only a few bacteria from milk (10⁰ cfu/100ml).
One method was that milk was cultured with an epual volume of twofold concentrated enrichment medium. Another was that milk added with hydrochloric solution was centrifuged to harvest coagulated milk (curd) as a sediment. The sedimented pellet was mixed with an equal volume of twofold concentrated enrichment medium, and cultured after the pH was adjusted to neutral with sodium hydroxide solution.