Concentrations of Selenium and Tocopherol in the Blood and Organs of Horses Administered with Large Dosis

Abstract

A high dosis of E·ESE (contains 2.5mg of selenium and 50mg of d-a-tocopherol in 1 ml) was injected intramuscularly into three horses and the changes of their concentrations in the blood and organs after administration were determined.
In a horse injected once with E·SE at 8.3ml/100kg, increases of from 149.8 to 225.3 ppb in serum selenium and from 676.8 to 1369.6μg/100ml in serum tocopherol were observed during a 6-day examination period, while no increase in activity was seen as regards blood glutathioneperoxidas.
In a horse injected twice at a 7-day interval with 8.6ml/100kg of E·ESE increases of from 146.0 to 369.9 ppb in serum selenium and from 544.6 to 1443.6μg/100ml in serum tocopherol were observed during a 2-week examination period, but no increase in the blood glutathioneperoxidase activity was seen.
In a horse received six injections at a 10 day interval with 5.72ml/100 kg of S·SE, increases of from 72.6 to 119.2 ppb in serum selenium and from 386.5 to 448.3μg/100ml in serum tocopherol were observed during the 60-day examination period. A slight increase in the blood glutathioneperoxidase activity was also observed after 40 days of administration. Selenium and α-tocopherol concentrations in the organs were determined 6 to 10 days after the termination of the experiment. The selenium concentrations in the liver, spleen and kidneys were higher than those of the control horses. The γ-tocopherol concentrations in the liver, adrenal glands, spleen, skeletal muscle and kidneys were higher than those of the control horses.
No clinical symptoms were observed in any of the horses examined.