1992 Volume 45 Issue 5 Pages 339-343
A simple quantitative method for the simultaneous determination of residual sulfa drugs in livestock products by high performance liquid chromatography (HPLC) was developed. The 12 sulfa drugs were sulfaguanidine (SGD), sulfadiazine (SDZ), sulfasetamaid (SCA), sulfamerazine (SMR), sulfadimidine (SDD), sulfamethoxypyridazine (SMPD), sulfachloropyridazine (SCPD), sulfamethoxazole (SMZ), sulfisoxazole (SIX), sulfadimethoxine (SDMX), sulfamonomethoxine (SMMX) and sulfisomidine (SID). Livestock products included pork, the liver, urine, serum, and kidney of swine, as well as chicken meat, cow milk and eggs.
Samples were deproteinized with trichloroacetic acid. Then, they were extracted with chloroform. The extracted supernatants from the samples were reacted with o-phthalaldehyde, and injected into an Inertsil C-8 (4mm I.D.× 250mm) HPLC column. Sulfa drugs were separated with a solution containing 1% acetic acid and acetonitrile as a mobile phase and detected with a spectrofluorometric detector (EX 285nm, EM 445nm).
The recoveries of drugs added to livestock products fortified at 100ng/g (ml) were from 70.0 to 168.0%. The detection limits were 2ng/ml for SMPD, 1ng/ml for SIX and SCPD, and 0.1ng/ml for other drugs. The fraction of (SDD and SDMX) that were picked by HPLC were able to react with the enzyme immunolinked analysis.
In an experimental SDD administration to swine, the SDD concentration in the muscles was about 10% of SDD concentration in the urine. Therefore, it was confirmed that the determination of SDD in the urine was a useful indicator for estimating the residual SDD concentration in muscles.
