Effects of Retinol and α-Tocopherol on Nitroblue Tetrazorium Reduction Ability of Neutrophils from Blood of Lactating Cows

Abstract

The effects of retinol and a-tocopherol on the bacteriocidal ability of neutrophils obtained from 20 lactating cows were investigated in vitro. After the separation of neutrophils from blood, the nitroblue tetrazorium (NBT)(NBT) reduction test was conducted under the conditions of no addition, retinol addition and atocopherol addition. The highest NBT reduction abilities were obtained at 100 IU/dl of retinol and at 200 μg/dl of a-tocopherol, respectively. This suggests that the bacteriocidal ability of neutrophils from lactatingcowsisinfluenced in vitro by thelevelsofretinol and α-tocopherol.