Abstract
Serum samples from jaundice cases of swine, bovine and equine were analyzed for conjugatedand unconjugated bilirubin (BL) using high-performance liquid chromatography (HPLC) with UV detection. A sharp HPLC peak of conjugated and unconjugated BL was obtained with a retention time of 4. 3 and 7.1 min respectively, using a simple mobile phase of methanol: 0.5MTris-HCl buffer (65: 35, pH 7.5) and a Nova-Pak C-18 column (3.9mm×150mm) at a flow rate of 0.7ml/min. The variable-wavelength detector was set at 450nm. The recovery rate of BL was 86.0%. The sera from animals with jaundice at a meat inspection site were analyzed by HPLC. In the suspected cases of obstructive jaundice, conjugated-BL was predominant; while the suspected cases with cholestatic jaundice were of the conjugated-BL predominant type or the intermediate type. Most of the suspected cases of hepatogenous jaundice were of the unconjugated-BL predominant type. Unconjugated-BL was predominant in all suspected cases of constitutional jaundice in equine. These equine had no lesions on their livers.
