The intraductal endoscopic examination was performed on bovine udders and teats in normal and mastitic cows, using a 0.75mm fiber-optic flexible endoscope and / or a 2.7mm rigid endoscope. Normal cases showed regularly arranged longitudial holds of the papillary duct, closed Rosettes, annular holds at the junction of the teat and gland sinuses. Submucosalvessels in the gland sinus were clearly observed and the whole surface of ductal mucous membrane was smooth and luster. Clinical and subclinical cases with mastitis showed hypertrophic and meandered holds of papillary duct, opened Rosettes, stenosed duct and irregularsurface of membrane. These findings suggest that the endoscopic examination is an useful technique for the evaluation of bovine mastitis.
The Lipid metabolism was investigated inHaemophilus somnusinfected cows associated with a high concentration of endotoxin. The LDL fraction of serum lipoproteins increased in all 3 cows. An increase in VLDL fraction was found in 2 of the 3 cows. These findings may suggest that the lipids metabolism impaired inHaemophilus somnusinfection.
Total anomalous pulmonary venous connection (TAPVC) was observed in 20 (5.3%) of 375 bovine hearts showing congenital cardiovascular anomalies. The ages at necropsy were 129 days or less, and only 4 cases survived over 2 months. The Pulmonary veins joined to form a common pulmonary vein above the atria and none of them were connected to the left atrium. The common pulmonary vein was connected to the left azygos vein, right azygos vein and coronary sinus in 11, 6 and 1 cases, respectively, while the site of anomalous connection of the common pulmonary vein was unknown in the remaining 2 cases. Thirteen cases were of isolated TAPVC without other cardiovascular anomaly with the patent foramen ovale as shunting channel for the left atrium, and many of them had enlarged and hypertrophic the right atrium and ventricle. Seven cases with associated cardiovascular anomalies showed various anatomic features in the heart.
Six flukes were detected from the cecum of Japanese black cattle raised at a farm in Esashi, Iwate Prefecture and identified asHomalogaster paloniaeby the morphological characteristics. Cercariae ofH. paloniaewere obtained from a snail, Polypylis hemisphaerulla collected from a rice paddy belonging to the farm. Furthermore, eggs ofH. paloniaewere detected from fecal samples of cattle reared at three farms in the same area. These results suggested thatH. paloniaeinfection was distributed in Tohoku District, Japan.
In 97 cats clinically diagnosed as hypertrophic cardiomyopathy (HCM) at Angell Memorial Animal Hospital in Boston, the occurrence of arrhythmia, congestive heart failure (CHF) and systemic arterial embolism (SAE) as well as heart rate and population characteristics wereevaluated, referring to electrocardiogram (ECG) and M-mode echocardiogram. The mean age ofcases with HCM was 5.3 years, and neutered males were significantly predisposed. The left ventricular free wall (LVFW) was significantly thicker in cats with SAE and in those showing left ventricular hypertrophy (LVH) pattern on ECG. It suggested that the LVFW thicknessmight be a closely related with SAE.
Serum samples from jaundice cases of swine, bovine and equine were analyzed for conjugatedand unconjugated bilirubin (BL) using high-performance liquid chromatography (HPLC) with UV detection. A sharp HPLC peak of conjugated and unconjugated BL was obtained with a retention time of 4. 3 and 7.1 min respectively, using a simple mobile phase of methanol: 0.5MTris-HCl buffer (65: 35, pH 7.5) and a Nova-Pak C-18 column (3.9mm×150mm) at a flow rate of 0.7ml/min. The variable-wavelength detector was set at 450nm. The recovery rate of BL was 86.0%. The sera from animals with jaundice at a meat inspection site were analyzed by HPLC. In the suspected cases of obstructive jaundice, conjugated-BL was predominant; while the suspected cases with cholestatic jaundice were of the conjugated-BL predominant type or the intermediate type. Most of the suspected cases of hepatogenous jaundice were of the unconjugated-BL predominant type. Unconjugated-BL was predominant in all suspected cases of constitutional jaundice in equine. These equine had no lesions on their livers.