1998 Volume 51 Issue 9 Pages 487-490
Bovine viral diarrhea virus (BVDV) 2 infection in cattle, swines, sheep, goat and deers was serologically surveyed in Ishikawa Prefecture. Only 1.1% of adult cattle had antibodies against BVDV 2, strain KZ-91, and retrospective serology with 1-year-old bovine sera collected since 1986, revealed that 1 of 70 cases of 1988, 2 of 51 cases of 1989 and 1 of 27 cases of 1994 had antibodies. Two of 10 BVDV isolates in 1990 were classified as BVDV 2, by restriction endonuclease analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) using a primer set to match the 5' untranslated region sequence of pestivirus.