Determination of Cephalosporin Antibiotics in Beef Meat using High-Performance Liquid Chromatography

Abstract

High-performance liquid chromatography (HPLC) was applied to determination of residual cephalosporins in beef meat. Five drugs, cefazolin, cefalonium, cefuroxime, cephapirin and ceftiofur, were added to meat samples (0.5μg (p)/g), which were extracted with 0.3% metaphosphoric acid-methanol (7: 3), and the extract was cleaned up on an Oasis HLB cartridge. The HPLC separation was carried out on an Inertsil ODS-3V column (4.6mm ID-250mm) with a mobile phase of 25mmol/l phosphate buffer (pH 2.8)-acetonitrile (75: 25) at a flow-rate of 1ml/min. The extracts were analyzed using a UV detector with time programming. The recoveries at levels of (0.5μg (p)/g) were 78.3 to 91.6%, and the detection limit of each drug was 0.05μg (p)/g.