Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Comparison between a New ELISA Kit and Virusneutralization Tests for the Detection of Antibodies of the Classical swinefever virus
Takashi NAKANEHiroaki WAKAMOTONaoyoshi ASHIZAWA
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2002 Volume 55 Issue 12 Pages 783-788

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Abstract
As antigen, the new ELISA kit developed for detecting antibodies of the Classical swinefever virus employs the E2 glycoprotein from the ALD-A 76 strain of the inactivated Classical swine-fever virus. We compared this new kit with virusneutralization (NT) tests using the CPK-NS cells and vaccinestrain GPE-. Both methods for detecting antibodies were used on 292 serum samples collected from suckling pigs, fattening pigs, and sows. Significant correlation (r= 0.66) was shown between the ELISA values and NT antibody titers. Degree of agreement between the ELISA and the NT tests was 91.1%. These results indicate that, in surveys for the Classical swinefever virus, the ELISA kit is useful in rapid serological screening of large numbers of samples.
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