As antigen, the new ELISA kit developed for detecting antibodies of the Classical swinefever virus employs the E2 glycoprotein from the ALD-A 76 strain of the inactivated Classical swine-fever virus. We compared this new kit with virusneutralization (NT) tests using the CPK-NS cells and vaccinestrain GPE-. Both methods for detecting antibodies were used on 292 serum samples collected from suckling pigs, fattening pigs, and sows. Significant correlation (r= 0.66) was shown between the ELISA values and NT antibody titers. Degree of agreement between the ELISA and the NT tests was 91.1%. These results indicate that, in surveys for the Classical swinefever virus, the ELISA kit is useful in rapid serological screening of large numbers of samples.
To evaluate cellular immunity in Japanese Black (JB) calves, changes in peripheral leucocytes were com-pared in two groups of calves–JB calves (JB group;n=10) and Holstein calves (Hol group; n=10). In both groups flow cytometric analysis (FCM) was used within 3 days (0 week) and 3, 6, 9, and 12 weeks after birth.Forward (FSC) and side-scatter (SSC) dot-plots were examined and used to construct monocyte-lymphocytes gates of 3 sizes (small, medium, and large). In both groups, numbers of medium (M)-ML were low in 0 week but increased after birth. In the JB group, however, numbers of M-ML decreased at from 6 to 9 weeks and were significantly lower than those in the Hol group from 6 weeks after birth. Numbers of small (S)-ML in the JB group peaked at 3 weeks and were significantly higher than in the Hol group at 0, 3, 9, and 12 weeks. FCM analysis showed that numbers of CD3+ cells in the JB group were significantly higher than those in the Hol group at 3 weeks after birth. These results indicate that cellular immunity in JB calves differs from that in Hol calves because of changes depending on the role of the T cell.
The effects of propionylpromazine(0.05mg/kg IV; PRP, n=30)were compared with those of acepromazine(0.05mg/kg; ACP, n=30)as premedication before inhalation anesthesia of dogs. Surgical anesthesia was induced with ketamine 5mg/kg IV and maintained with 50% nitrous oxide-50% oxygen-sevoflurane. After pre-medication, all dogs became calm and relaxed and demonstrated moderate sedation. After injections of keta-mine, convulsions occurred in 1 dog in the ACP group and in 2 dogs in the PRP group. After induction of anes-thesia, apnea occurred in 8 dogs in the ACP group and 10 dogs in the PRP dogs. Controlled ventilation was required to improve ventilation and/or to maintain surgical anesthesia in 7 dogs in the ACP group and 4 dogs in the PRP group. End-tidal sevoflurane concentration during surgery varied between 2.0 and 2.2%. During surgery, body temperatures dropped below 36 t in 12 dogs in the ACP group and in 13 dogs in the PRP group. Hypothermia(<35.0°C)developed in 3 dogs in each group. Throughout surgery in both groups, heart rate, respiratory rate and partial pressure of end-tidal CO2were maintained within normal canine limits: 120 bpm, 25 bpm, and 40mmHg, respectively. Hypotension occurred in 6 dogs in each group. Recovery from anesthesia was rapid, and most dogs were extubated 10 minutes after anesthesia cessation. No differences between the premedication effects of propionylpromazine and acepromazine were apparent. Both are useful as premedica-tion for dogs.
A new urinary-diversion technique was performed to bypass a Stenotic portion of the urethra of a 9-year-old bitch. The urethra, resected in the pelvic visceral cavity, was led outside the abdominal cavity at the forward edge of the prepubis. The urethra was then passed through the ventral side of the pubic symphysis, and its cut end was directed towards the vagina. Finally, it was passed into the vagina, where a new urethral meatus was created in the vaginal floor. Post-surgical continence was maintained, and no complications were associ-ated with the procedure.