An In Vitro Chemosensitivity Test against Canine Mammary gland Tumors Using Collagen Gel Droplet Embedded Cultures

Abstract

Our basic study on the usefulness of the collagen gel droplet embedded culture drug-sensitivity test (CDDST) showed that canine mammarygland tumor cells can be cultured well in collagen gel droplets. With this method, we employed five kinds of anticancer drugs-adriamycm, 4OH-cyclophosphamide, vincristine, 5-fluorouracil, and predonisolone-against tumor cells. An image-analysis system was used to analyze their in vitro anticancer activities. We found that anti-cancer drug efficacy against canine mammary gland tumors differed from dog to dog. The CD-DST method can be applied to canine tumors and may be an index for so-called custum-made therapy.