Morphological and Molecular Characterization of Explanatum Explanatum from Cattle and Buffaloes in Myanmar

Madoka ICHIKAWA; Daisuke KONDOH; Saw BAWN; Ni Ni MAW; Lat Lat HTUN; Myint THEIN; Aung GYI; Kyaw SUNN; Ken KATAKURA; Tadashi ITAGAKI

doi:10.1292/jvms.12-0389

Parasitology

Morphological and Molecular Characterization of Explanatum Explanatum from Cattle and Buffaloes in Myanmar

Madoka ICHIKAWA, Daisuke KONDOH, Saw BAWN, Ni Ni MAW, Lat Lat HTUN, Myint THEIN, Aung GYI, Kyaw SUNN, Ken KATAKURA, Tadashi ITAGAKI

Author information

Madoka ICHIKAWA
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi–2–13 Inada-cho, Obihiro, Hokkaido 080–8555, Japan
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan
Research Fellow of the Japan Society for the Promotion of Science
Daisuke KONDOH
Department of Basic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Saw BAWN
Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Naypyidaw 05282, Myanmar
Ni Ni MAW
Livestock Breeding and Veterinary Department, Insein, Yangon 11011, Myanmar
Lat Lat HTUN
Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Naypyidaw 05282, Myanmar
Myint THEIN
Livestock Breeding and Veterinary Department, Insein, Yangon 11011, Myanmar
Aung GYI
Livestock Breeding and Veterinary Department, Insein, Yangon 11011, Myanmar
Kyaw SUNN
Livestock Breeding and Veterinary Department, Insein, Yangon 11011, Myanmar
Ken KATAKURA
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 8, Sapporo, Hokkaido 060–0818, Japan
Tadashi ITAGAKI
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan

Keywords: amphistome, Explanatum, ITS2, molecular marker, morphological characterization

JOURNAL FREE ACCESS

2013 Volume 75 Issue 3 Pages 309-314

DOI https://doi.org/10.1292/jvms.12-0389

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Abstract

A robust molecular marker is needed for discrimination of amphistome species, because identification based on morphology alone requires specialized knowledge and techniques. In this study, we performed morphological and molecular characterization of Explanatum explanatum, a species that causes severe liver damage in definitive host species. Fifty-five adult amphistomes were collected from cattle and water buffaloes in Myanmar. Eighteen of the amphistomes, arbitrarily chosen, were morphologically identified as E. explanatum using sagittal sections. All of the 55 amphistome isolates had identical second internal transcribed spacer (ITS2) of ribosomal DNA sequences; these sequences differed at 7 nucleotide sites from those of the closest species, Paramphistomum leydeni. Our data indicate that the ITS2 sequence could be a useful molecular marker for epidemiological studies on E. explanatum.

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